The BioOptiX project is an intelligent, multi-module system for real-time monitoring, analysis, and optimization of bioreactor processes. The software is designed by integrating multi-objective optimization algorithms (such as NSGA-II), machine learning models, digital twins, and interactive graphics systems. Users can monitor system performance in real time and extract the most optimal operating conditions by importing process data or connecting to live data from PLCs. BioOptiX is a flexible, scalable tool that can be used in academic research, bioindustries, and innovation centers.